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Image Search Results
Journal: Journal of Virology
Article Title: Sequence Analysis of In Vivo Defective Interfering-Like RNA of Influenza A H1N1 Pandemic Virus
doi: 10.1128/JVI.00240-13
Figure Lengend Snippet: Detection of DI-like RNAs in clinical specimens. (A and B) RNA extracted from selected INSIGHT clinical specimens (INS002 to INS036) was subjected to PB2 segment-specific RT-PCR using SuperScript III (SSIII) first-strand synthesis kit MLV RT (Invitrogen) (A) or AMV RT (New England BioLabs) (B). INS036 is a negative control. The positions of molecular size standards (in kilobases) are shown to the left of the gels.
Article Snippet: This supports our hypothesis that DI-like RNAs are real and the observed short segments in the sequence data are not the result of reverse transcriptase movement or Taq errors during M-RT-PCR. fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window Fig 5 caption a7 Detection of DI-like RNAs in clinical specimens. (A and B) RNA extracted from selected INSIGHT clinical specimens (INS002 to INS036) was subjected to PB2 segment-specific RT-PCR using SuperScript III (SSIII) first-strand synthesis kit MLV RT (Invitrogen) (A) or
Techniques: Reverse Transcription Polymerase Chain Reaction, Negative Control